Hi Zq Deng

No-one has mentioned microseeding into random screens, the so-called Microseeding Matrix Screening (MMS) method , or just “matrix seeding”.

This is exactly the kind of situation where the method works really well.  You will very often get many new leads, as well as bigger crystals.

See the excellent paper by D’Arcy et al., Acta Cryst. (2007). D63, 550–554, and also the original paper of Ireton and Stoddard, Acta Cryst. (2004). D60, 601-605.  See also the practical and theoretical comments at http://www.douglas.co.uk/mms.htm

Best wishes

Patrick

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From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of zq deng
Sent: 06 May 2010 09:04
To: [log in to unmask]
Subject: [ccp4bb] control of nucleation

hello,everybody . due to excess nucleation,I often get many tiny crystals instead of  few,large crystals.i wana optimize the condition, does anyone have adivce about this?  

Best regards.