 |
 |
Dear Zq, A few ideas: 1) Vary protein concentration, temperature, or protein : mother liquor ratio. 2) Try dioxane - it is supposed to reduce nucleation. 3) give your protein a good hard spin before you set up drops to remove aggregates. 4) seeded factorial screen. 5) re-purify on gel filtration? Ed ______________ T.Edwards Ph.D. Garstang 8.53d Astbury Centre for Structural Molecular Biology University of Leeds, Leeds, LS2 9JT Telephone: 0113 343 3031 http://www.bmb.leeds.ac.uk/staff/tae/ -- Nature composes some of her loveliest poems for the microscope and the telescope. ~Theodore Roszak ________________________________ From: zq deng <[log in to unmask]> Reply-To: zq deng <[log in to unmask]> Date: Thu, 6 May 2010 09:03:46 +0100 To: <[log in to unmask]> Subject: [ccp4bb] control of nucleation hello,everybody . due to excess nucleation,I often get many tiny crystals instead of few,large crystals.i wana optimize the condition, does anyone have adivce about this? Best regards.