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Barring some grammatical errors, you've pretty much summed it up.

James


On May 18, 2010, at 12:31 AM, Vinson LIANG wrote:

> Dear all,
>
> Sorry for this silly biochemistory question.  Thing is that I have a  
> reversible epimerase and I want to mutate it into an inreversible  
> one. However, I have been told that the ΔG of a reversible reaction  
> is zero. Which direction the reaction goes depends only on the  
> concentration of the substrate.  So the conclusion is,
>
> A: I can mutate the epimerase into an inreversible one. But it has  
> no influence on the reaction direction, and hence it has little mean.
>
> B: There is no way to change a reversible epimerase into an  
> inversible one.
>
> Could somebody please give me some comment on the two conclution?
>
> Thank you all for your time.
>
> Best,
>
> Vinson
>
>