Dear Arpita,
For Getting rid of DNA you can incubate the lysate with DNase and incubate it before lysing  the cells with sonication or french press (or anyother method of your choice)

cheers
Rashmi


On Wed, May 12, 2010 at 8:53 PM, Arpit Mishra <[log in to unmask]> wrote:


---------- Forwarded message ----------
From: Arpit Mishra <[log in to unmask]>
Date: Wed, May 12, 2010 at 6:20 PM
Subject: regarding purification of DNA binding protein
To: [log in to unmask]


hi all
i am trying to purify DNA binding protein, but i couldnt get rid of the DNA after performing hi-trap heparin column, i have also tried high conc salt buffer (upto 1 M NaCl) and also tried o.2 M lithium sulphate, please suggest me some way to get rid of undesired DNA..

thanks

Regards

Arpit





--
rashmi