HiOn 19 Apr 2010, at 20:13, Klara Mareckova wrote:Hi,could anybody please help me setting up my design for a voxelwise analysis of 2by2 ANOVA? I'm trying to follow the directions from http://www.fmrib.ox.ac.uk/fsl/feat5/detail.html, but I'm not really sure if I'm setting up the EVs correctly.My study has 40 subjects divided into 4 groups: on pill & menstruation, on pill & ovulation, free & menstruation, free & ovulation.In the first level analysis, I had 5 conditions (EVs) where participants were were watching angry faces, angry hands, neutral faces, neutral hands or control condition. In the higher level analysis, I would like to explore the main effect of pill, main effect of cycle phase and their interaction.Setting up the contrasts & F test seems pretty easy then:pill 100 0 on, off, offcycle 010 0 off, on, offpill * cycle 001 0 off, off, onWhat is more of a puzzle for me are the EVs:1. Am I not working with my 5 conditions from 1st level analysis (angry, neutral,..) at all?I don't quite understand - presumably at first level you will make a contrast of interest (e.g. angry vs neutral) and then feed that up for all sessions/subjects into the higher level modelling? You could then repeat that for each first-level contrast of interest?Yes2. If so, would the EV set up look like this?group cycle group interaction grandmean1 1 1 1 11 1 1 1 11 1 1 1 11 1 1 1 11 1 1 1 11 -1 1 -1 11 -1 1 -1 11 -1 1 -1 11 -1 1 -1 11 -1 1 -1 12 1 -1 -1 12 1 -1 -1 12 1 -1 -1 12 1 -1 -1 12 1 -1 -1 12 -1 -1 1 12 -1 -1 1 12 -1 -1 1 12 -1 -1 1 12 -1 -1 1 13. With the set up above, I'm getting a warning: "design matrix uses different groups but these do not separable EVs for the different groups"yes - this design is not "separable" so just set all the "group memberships" to 1.Cheers.Do you happen to have any suggestion what am I supposed to change to get the right design for my study?Thanks a lot,Klara
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Stephen M. Smith, Professor of Biomedical Engineering
Associate Director, Oxford University FMRIB Centre
FMRIB, JR Hospital, Headington, Oxford OX3 9DU, UK
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