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What space did you use as seed space? If you used native diffusion space, you can simply threshold the pathway (fslmaths <image> -thr <threshold> <thresholded pathway>). Then fslstats <FA> -k <thresholded pathway> -M to get the mean value across the pathway. If you used some other space, you would need to use FLIRT to transform the results back into native diffusion space. Peace, Matt. -----Original Message----- From: FSL - FMRIB's Software Library [mailto:[log in to unmask]] On Behalf Of Emanuele Tinelli Sent: Sunday, April 04, 2010 5:35 AM To: [log in to unmask] Subject: [FSL] FA e MD value on single voxel track Hi, I followed FDT process; how can i get the FA and MD value of a track (fibers) created with single-voxel created with probtrackx? Thanks