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Amit Sharma, I second Tim's suggestions. The first experiment is the following. Set up several experiments at constantly decreasing precipitant concentrations. Streak seed all of them. If in any of those you can get non-clustered crystals non matter what size, seeding can solve your problem. To restore crystal size follow the rest of Tim's list. Enrico. On Mon, 12 Apr 2010 23:01:10 +0200, Tim Gruene <[log in to unmask]> wrote: > Have you tried anything, yet? The list of answers can be quite long. > A couple of keywords: > - micro-seeding > - macro-seeding > - cross-seeding > - additive screens > - extend to purification protocol > - change temperature, pH, etc. > - have you searched around the current conditions > ... > > if you describe a little what you have done so far we can also get an > idea of > your status of experience and adapt the answers accordingly. > > Kind regards, Tim -- Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html e-mail: [log in to unmask] Fax: 33 (0)1 69 08 90 71