Dear all,

I already received a couple of replies.

* mammalian cells:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.pdf
mentions several mammalian cell lines, including CHO, HEK239, and COS.
Apparently, Lec8 have been used, too.
* Yeast
* stable S2 insect cells
* baculovirus expression system
* in addition to E.coli, P. aeruginosa was also mentioned.

Thanks to everyone who replied.

Tim


On Tue, Mar 23, 2010 at 01:25:56PM +0100, Tim Gruene wrote:

Dear all,

since I am currenty preparing a lecture on crystallography I am wondering about

the status quo of the production of SeMet proteins.

In 2003, if I remember correctly, it was possible to express SeMet proteins in

E.coli and insect cells.

Has this been extended to other systems, and if so, which ones?

Thanks a lot,

Tim

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Tim Gruene

Institut fuer anorganische Chemie

Tammannstr. 4

D-37077 Goettingen

GPG Key ID = A46BEE1A

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Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen

GPG Key ID = A46BEE1A