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I think your crystallographic colleague is misled. SCALEIT simply scals two or more sets of ampiltudes to the chosen one. ( labelled FPH1 FPH2 etc scaled to FP..) It knows nothing of the contents of the asymmetric unit.. truncate uses the number of residues in the asymmetric unitr to assign a rough scale to the amplitudes - in that case if you estimated there were 2 molecules per asymm unit and there were actually three it would not be correct. Eleanor Francois Berenger wrote: > Graeme Winter wrote: >> Hi Francois, >> >> SCALEIT in CCP4 sounds like the tool you want - this is for scaling >> e.g. native and derivitive data sets together. You will need to cad >> together the two files first though. > > My crystallographer colleague tells me that if we use > scaleit there is a risk if there are multiple copies in the ASU. > > So, we should scale both data sets to absolute scale. > >> This is illustrated in the tutorials here: >> >> http://www.ccp4.ac.uk/dist/examples/tutorial/html/heavy-tutorial-mad.html#step_4a >> >> >> Best wishes, >> >> Graeme >> >> On 12 March 2010 08:17, Francois Berenger <[log in to unmask]> wrote: >>> Hello, >>> >>> Is there a magic tool doing the job of scaling 2 MTZ to the same scale? >>> >>> For the moment I know with ccp4: >>> rwcontents then wilson then mtzutils >>> >>> with phenix: >>> lsq_scale (in fact I am lying, I was forced to run ccp4's cad before) >>> >>> Is there a simpler way with ccp4? >>> As I am not a crystallographer, I am afraid I can do many >>> different stupid errors when I have to use many tools for just >>> one task. >>> >>> Regards, >>> Francois. >>>