You can do either way, but if you run it on the whole series you will need to make bvecs file and a bvals file that includes all of the volumes. Peace, Matt. -----Original Message----- From: FSL - FMRIB's Software Library [mailto:[log in to unmask]] On Behalf Of Ed Gronenschild Sent: Monday, January 25, 2010 10:20 AM To: [log in to unmask] Subject: [FSL] Is averaging of DTI dat necessary? Hi, I have a set of 6 series of DTI data, each consisting of 13 volumes, with volume 1 referring to B=0 and volumes 2-13 referring to 12 different B vectors. My question is: should I average the 6 series (after correction of eddy current and movement) before running dtifit or can I just run dtifit on the whole set of 78 volumes? Kind regards, Ed