Look into AKRAprime. It’s good for
low pressure columns (Max 1MPa) and costs ~$14,000 with accessories. For the
price of a FPLC, we have several AKTAprime for running SEC columns. --Chun
From: CCP4 bulletin
board [mailto:[log in to unmask]] On
Behalf Of Sangeetha Vedula
Sent: Thursday, January 28, 2010
8:41 AM
To: [log in to unmask]
Subject: Re: [ccp4bb] Biorad
Profinia vs GE AKTAPrime Plus, for affinity tagged proteins
Thanks for your response,
Edward. Yes, that is what I thought. For the most part, people plan to tag
their proteins in my lab. I'll possibly be the only one purifying non-tagged
proteins in my lab. I love the AKTA. Another was all praise of the Profinia. To
my knowledge, tagged proteins don't always get fully purified with just an
affinity column. An additional size exclusion step helps. That is, if the tag is
even available at the surface for binding the affinity column. My vote is AKTA.
So, I wanted to find out what the world thought about both.
On Thu, Jan 28, 2010 at 11:16 AM, Edward Collins <[log in to unmask]>
wrote:
personally, I don't know why you would want to limit yourself to just
using affinity tags to purify your proteins. For my money the flexibility
of the AKTA is critical.
good luck
-ed
On Jan 28, 2010, at 11:08 AM, Sangeetha Vedula wrote:
> Dear all,
>
> My lab is looking into buying a protein purification system. The AKTA is
more versatile, but does anyone know if the Profinia is in any way superior to
the AKTA for purification of affinity-tagged proteins? If it is, I would really
appreciate if you can tell me in what way it is superior.
>
> Thanks,
>
> Sangeetha.