When working on tobacco Rubisco in Los Angeles, we got the
best crystals of the activated form of the protein after small earthquakes (4-5
on the richter scale). However, as people in the lab. noticed, this earthquake
parameter is somewhat difficult to control. Handling the linbro plates sometimes
caused massive nucleation: I would look at a plate with only clear drops and by
the time I would put the plate back in the incubator there was so much
nucleation that the plate was of no use any more. When the nucleation of your
protein is sensitive to vibrations, I would worry more about handling than about
the fire alarm.
Best,
Herman
Dear all,
Does anyone know the effect of a fire alarm placed in the same room where
one grows crystals?
(1) On trays placed inside styrofoam boxes
(2) On trays placed inside an incubator which has not been tested as
vibration free.
Thank you.
Jackie Vitali