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Look into AKRAprime. It's good for low pressure columns (Max 1MPa) and costs ~$14,000 with accessories. For the price of a FPLC, we have several AKTAprime for running SEC columns. --Chun _____ From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Sangeetha Vedula Sent: Thursday, January 28, 2010 8:41 AM To: [log in to unmask] Subject: Re: [ccp4bb] Biorad Profinia vs GE AKTAPrime Plus, for affinity tagged proteins Thanks for your response, Edward. Yes, that is what I thought. For the most part, people plan to tag their proteins in my lab. I'll possibly be the only one purifying non-tagged proteins in my lab. I love the AKTA. Another was all praise of the Profinia. To my knowledge, tagged proteins don't always get fully purified with just an affinity column. An additional size exclusion step helps. That is, if the tag is even available at the surface for binding the affinity column. My vote is AKTA. So, I wanted to find out what the world thought about both. On Thu, Jan 28, 2010 at 11:16 AM, Edward Collins <[log in to unmask]> wrote: personally, I don't know why you would want to limit yourself to just using affinity tags to purify your proteins. For my money the flexibility of the AKTA is critical. good luck -ed On Jan 28, 2010, at 11:08 AM, Sangeetha Vedula wrote: > Dear all, > > My lab is looking into buying a protein purification system. The AKTA is more versatile, but does anyone know if the Profinia is in any way superior to the AKTA for purification of affinity-tagged proteins? If it is, I would really appreciate if you can tell me in what way it is superior. > > Thanks, > > Sangeetha.