You really don't need much of a system to do just
affinity purification, so almost anything would be workable unless you
are doing really high throughput work. On the other hand, if you need
to do traditional protein purification (IEX, HIC, GEC), which would
include untagged proteins, and polishing of tagged or untagged
proteins, then a good chromatography system is a must. I've owned,
used, and abused an AKTA FPLC since 1999. I think I have one of the
first units. It is built like a tank, and is still expandable. We just
upgraded the computer system and associated hardware to a modern OS
(Win NT4 to XP). I don't know about the Profina units, but the
AKTA system can take daily abuse with aplomb. I't been in a cold room
for over 10 years and it's still going.
Cheers.
On 1/28/2010 11:08 AM, Sangeetha Vedula wrote:
[log in to unmask]"
type="cite">Dear all,
My lab is looking into buying a protein purification system. The AKTA
is more versatile, but does anyone know if the Profinia is in any way
superior to the AKTA for purification of affinity-tagged proteins? If
it is, I would really appreciate if you can tell me in what way it is
superior.
Thanks,
Sangeetha.
--
Roger S. Rowlett
Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: [log in to unmask]
