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First you need to establish if it is your cryo conditions or the crystals. Depending where you are - they might have the equipment to do a wet mount - without freezing. Yes the crystal will not last - but then you know if the problem is in the crystal. If it is - you need better crystals. If it is the cryo - you need to work on that. Tacsimate is mixture of alot of different compounds - but the smears are too close together to be a small salt crystal on top... Good luck, Ezra On 1/26/2010 10:42 AM, Zhiyi Wei wrote: > Dear all, > > I got a problem with my crystals. I have two total different proteins > that both can be crystallized in the condition with PEG3350 and Tacsimate > (although the concentrations are different) with different shapes. The > crystals look big and beautiful. However, when I test them in synchrotron, > both of these two types of crystals showed poor diffractions. As showed in > the attached diffraction image, the diffraction is up to ~4 A but smear in > one direction while<8 A in the other direction. The interesting thing is > that the diffraction pattern is similar for all crystals (from two different > proteins) that I tested without exception although they belong to different > space groups. So, I wonder whether these kind of pattern is caused by > Tacsimate (I don't know what it is) and how to rescue these crystals. Any > suggestions or comments? > > Thanks a lot! > > Best, > Zhiyi >