Dear all,

I am solving a structure containing 2 heterodimers in one ASM. First, got the solution by MR( 11-3.6Å, modified model template with Rfac and Rfree 47% and 48%,respectively). After some model building by hand in terms of 2mFo-DFc map bearing 0.41 for Rfac and 0.42 for Rfree, except molecule 1,2 and 3 fitting the 2mFo-DFc map well , the 4^th molecule presenting poor, however, the 4^th molecule fit the mFo map well( of course, another 3 mols fit well). After model building by hand based on the mFo map coupled with a cycle of simulating and minimizing, it produced poor maps (2mFo-DFc as well as mFo, almost main chain broken, disorderly)  for mol 4 except molecule 1,2 and 3 fitting the 2mFo-DFc as well as mFo map well and just a little decreasing on Rfac and Rfree.

How can I do? Can anybody give me some inspiration?