Dear All checking out the Lysozyme crystallization methods on the web I liked the Rigaku Instructions that I found: (http://www.rigaku.com/protein/crystallization.html) "...create a drop of 3ul lysozyme solution, and 3 ul of well solution, respectfully, for a total drop size of 6ul..." So perhaps sometimes I am just not respectful enough to deserve crystals ? good wishes to all regards, Martyn ----------------------- Martyn Symmons MRC-MBU Cambridge UK 'Chan fhiosrach mur feòraich.' Gaelic proverb - Nothing asked, nothing learned.