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Dear All
      checking out the Lysozyme crystallization methods on the web I liked the Rigaku Instructions that I found:
(http://www.rigaku.com/protein/crystallization.html)

"...create a drop of 3ul lysozyme solution, and 3 ul of well solution, respectfully, for a total drop size of 6ul..."

So perhaps sometimes I am just not respectful enough to deserve crystals ?

    good wishes to all 
       regards,
         Martyn 
-----------------------
Martyn Symmons
MRC-MBU Cambridge UK 
'Chan fhiosrach mur feòraich.'
Gaelic proverb -
  Nothing asked, nothing learned.