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| Hi all, In one of my 1.6Ang resolution structure, I came across a strange density. I tried to model this with PEG but after refinement still peaks appear around PEG at ~1.45-1.7 Ang. The images before and after PEG insertion is at: http://mole.mbfys.lu.se/~saraboji/peg_density.jpg (Fo-Fc and 2Fo-Fc is at 3 and 1sigma) I feel this to be a PEG modification, which I could not resolve. My crystallization condition contains PEG, Sodium acetate, and Zinc chloride. Does anyone have experience in resolving such a density? Thanks in advance With best regards Saraboji --------------------------------- Dr. SARABOJI KADHIRVEL Post-Doctoral Researcher, Molecular Biophysics Center for Molecular Protein Science, Kemicentrum, Lund University, Box 124, SE-221 00 Lund, Sweden Tel: +46-46-2221448 (off); +46-76-2374172 (mob), Fax: +46-46-2224692; e-mail: [log in to unmask] |