Apologies for an off topic question. I was hoping for some advice regarding the use of sulfo-NHS diazirine
crosslinker (Pierce link) for in vitro protein interaction analysis.
Have
tried labeling the protein (9kDa, 10 to 20uM conc) with 2 primary
amines using 30-40X excess Sulfo-NHS-Diazirine crosslinker in PBS (1hour at 4deg). Have tried
other concentration combination as well, but this ratio yields some
usable material. However, majority of the protein crashes out of
solution.
Was wondering if anyone has any suggestions in optimizing the yield of crosslinker labelled protein?
Thanks in advance.
--
Rohan Agashe
