Hello, I have for some time now been linking root resonances in the HSQC spectrum to resonances in the backbone spectra. First I picked the obvious peaks in the HSQC and used the Assignment : Initialise Root resonances. Then I navigated from each root to peaks in the backbone spectra using Assignment : Pick & assign from roots. After doing this I found out that I had missed some peaks in the HSQC. I then picked the new peaks in the HSQC and assigned them to a new spin-system. Again I then navigated to the positions in the backbone spectra and assigned the peaks to that spin-system. But somethings has gone wrong, becuase yesterday I wanted to export my work to MARS and only the spin-systems created in the first run (with the Assignment : Initialise Root resonances command first) was exported. For some reason the program does not know that the remaining spin-systems are of the same "kind" as the first. Also I am confused about the assignment of C-atoms, because the spin-systems from the first run seems to have had the C-1 -atoms assignmed to a hole new spin-system, whereas the C-atoms dont have any assignment of the C-atoms. It seems very confusing to me and I dont know how to fix this. I dont know how to "tell" the program that the spin-systems of the second run is of same importance as those in the first. I hope you can help me and please write if you need more information. Regards, Gitte Haxholm ps. The assignment of C-1 -atoms from the spin-systems of first run to new spin-systems does not seem to be consistent. Some are assigned to new spin-systems and others are not. The ones that are not does not get exported and I have tried assigning them to a new spin-system my self, but it does not help.