Another way can use Iodination of tyrosines. You can use N-Iodo succinamid on existing crystals or on protein prior to crystallization.
Raz ----------------------------------------------------
Raz Zarivach, Ph.D.
Department of Life Sciences and the NIBN
Ben-Gurion University of the Negev
POB 653
Zip code 84105
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________________________________
From: Jacob Keller <[log in to unmask]>
To: [log in to unmask]
Sent: Mon, October 26, 2009 10:06:35 PM
Subject: Re: [ccp4bb] SAD phasing at home source
Or you can try also substituting anomalous cations into the mix, such as CsI, take multiple data sets, and do MIRAS:
NaCl
NaI
CsCl
CsI
One would then be able to subtract the various differences to find the HA's. Cs has a pretty good anomalous signal at CuKa wavelength (~8 electrons).
Jacob
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [log in to unmask]
*******************************************
----- Original Message -----
>From: James Murphy
>To: [log in to unmask]
>Sent: Monday, October 26, 2009 2:15 PM
>Subject: Re: [ccp4bb] SAD phasing at home source
>
>
>I have had success with Iodine, soak your crystal in your cryo + Potassium Iodide
>
>
>
>
________________________________
>From:CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of james09 pruza
>Sent: Monday, October 26, 2009 2:33 PM
>To: [log in to unmask]
>Subject: [ccp4bb] SAD phasing at home source
>
>Dear All,
>
>I need some suggestions regarding the SAD phasing using home source. What the the most commonly used HA for SAD phasing for Cu anode.
>All suggestions are welcome.
>Thanks.
>
>James
