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Or you can try also substituting anomalous cations into the mix, such as CsI, take multiple data sets, and do MIRAS: NaCl NaI CsCl CsI One would then be able to subtract the various differences to find the HA's. Cs has a pretty good anomalous signal at CuKa wavelength (~8 electrons). Jacob ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: [log in to unmask] ******************************************* ----- Original Message ----- From: James Murphy To: [log in to unmask] Sent: Monday, October 26, 2009 2:15 PM Subject: Re: [ccp4bb] SAD phasing at home source I have had success with Iodine, soak your crystal in your cryo + Potassium Iodide ------------------------------------------------------------------------------ From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of james09 pruza Sent: Monday, October 26, 2009 2:33 PM To: [log in to unmask] Subject: [ccp4bb] SAD phasing at home source Dear All, I need some suggestions regarding the SAD phasing using home source. What the the most commonly used HA for SAD phasing for Cu anode. All suggestions are welcome. Thanks. James