Dear Atul,

 

A question that any prudent structural biologist should ask sooner or later once ‘very tiny crystals’ are obtained is – are these crystals made of my protein of interest? I’ve witnessed at least three cases of ‘tiny crystals’ being another (unintended) protein (confirmed by solving the structure of the tiny crystals!) and a number of cases where tiny crystals turned out to be some unexpected kind of small molecule.

 

Good luck,

Artem

 

 

hi all
sorry for asking non ccp4bb question.
i am trying to crystallise  phosphatase protein,it has histag.I am getting very tiny crystals into .1m hepes ph7.4,1m Na-K tartrate.I tried additives(MPD,ethanol,propanol)for improvement of crystals,but didnt get any success.I have tried both sitting and hanging drop conditions.Does anyone have suggestion for the improvement of these crystals?
thanks
Atul Kumar

-----Original Message-----
From: CCP4 bulletin board on behalf of Jessica Gilmore
Sent: Wed 9/30/2009 2:44 AM
To: [log in to unmask]
Subject: [ccp4bb] Bioinformatics Technician Position - Center for the Study of Systems Biology, Georgia Tech

Bioinformatics Technician Position Available - Center for the Study of 
Systems Biology, Georgia Tech

Job Summary
Assists research staff in the integration, management and analysis of 
biological data, with a focus on the development, updating and 
maintenance of internal and public databases dealing with genome, 
transcriptome, proteome and metabolome annotation results generated in 
silico.

Job Qualifications
Requires a BS, MS, or PhD in Bioinformatics, Computer Science, or 
related fields.
Minimum of two years verifiable experience in web site development and 
database design.
Experience in scripting languages, preferably in a Unix/Linux 
environment.
Familiarity with the use of biological databases.
Demonstrated written and verbal communication skills.



To apply please email your CV to: [log in to unmask]