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Dear All, My protein has been reductively methylated, and experimental density shows that lysines and N-terminal amines are methylated, while its is easy to model methylated lysine in coot, I can hardly find a way to model the methylated N-terminal. Does anyone know how to do this? Will the methylated N-terminal amine be OK for Refmac refinement? Thanks in advance for any suggestions! ---------------------- Xiaoli Xiong PhD Candidate Department of Cellular and Molecular Medicine School of Medical Sciences University of Bristol University Walk Bristol BS8 1TD, UK [log in to unmask]