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It will help more if we send it to James ;)


On Oct 11, 2009, at 9:15 AM, gauri misra wrote:

> Dear James,
>
> As there are indications of protein degradation that have been  
> suggested in
> previous postings, i think adding some protease inhibitors right at  
> the
> stage of purification may provide some help.
>
> Moreover, you can also try various additives as specified in one of  
> the
> Hampton screens (certainly if the protein requires some cofactor for  
> its
> functioning that would be the priority as an additive).
>
> You have just given one condition. Is it that crystals are visible  
> only in
> this particular condition?
> If there are positive results in some other condition we can think of
> playing around that too as an alternative strategy.
>
> Hope this little piece of suggestion may help you at some point.
>
> Best wishes
>
> Gauri