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Dear Atul, A question that any prudent structural biologist should ask sooner or later once 'very tiny crystals' are obtained is - are these crystals made of my protein of interest? I've witnessed at least three cases of 'tiny crystals' being another (unintended) protein (confirmed by solving the structure of the tiny crystals!) and a number of cases where tiny crystals turned out to be some unexpected kind of small molecule. Good luck, Artem _____ From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of atul kumar Sent: Wednesday, September 30, 2009 1:58 AM To: [log in to unmask] Subject: [ccp4bb] improvement of tiny protein crystals hi all sorry for asking non ccp4bb question. i am trying to crystallise phosphatase protein,it has histag.I am getting very tiny crystals into .1m hepes ph7.4,1m Na-K tartrate.I tried additives(MPD,ethanol,propanol)for improvement of crystals,but didnt get any success.I have tried both sitting and hanging drop conditions.Does anyone have suggestion for the improvement of these crystals? thanks Atul Kumar -----Original Message----- From: CCP4 bulletin board on behalf of Jessica Gilmore Sent: Wed 9/30/2009 2:44 AM To: [log in to unmask] Subject: [ccp4bb] Bioinformatics Technician Position - Center for the Study of Systems Biology, Georgia Tech Bioinformatics Technician Position Available - Center for the Study of Systems Biology, Georgia Tech Job Summary Assists research staff in the integration, management and analysis of biological data, with a focus on the development, updating and maintenance of internal and public databases dealing with genome, transcriptome, proteome and metabolome annotation results generated in silico. Job Qualifications Requires a BS, MS, or PhD in Bioinformatics, Computer Science, or related fields. Minimum of two years verifiable experience in web site development and database design. Experience in scripting languages, preferably in a Unix/Linux environment. Familiarity with the use of biological databases. Demonstrated written and verbal communication skills. To apply please email your CV to: [log in to unmask]