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Dear Jack I can certainly recommend using the litholoops from molecular dimensions to mount the crystal you descibe. Most of the membrane crystal we start out with come as needles, the advantages is as mention before that the slight twist in the nylon loops isn't present. We do not have much success with the litholoops looking like little rackets. best Preben Tanner, John J. wrote: > Dear CCP4, > > I'm looking for advice on mounting thin needles for low temperature data > collection. Our needles are fairly long (100-200 microns) but only 20 > microns or less thick. When I pick them up with Hampton loops (0.05-0.1 mm > size), the crystals tend to break as they are moved out of the drop and > through the liquid-air interface. > > I see that Mitegen sells MicroLoops E, which are advertised as working well > for mounting needles. Can anyone recommend them? Can anyone recommend > Mitegen MicroMeshes or another tool for mounting needles? > > Thanks, > > Jack Tanner > > > -- Jens Preben Morth, Ph.D Aarhus University Department of Molecular Biology Gustav Wieds Vej 10 C DK - 8000 Aarhus C Tel. +45 8942 5025, Fax. +45 8612 3178 [log in to unmask] website: http://person.au.dk/da/jpm@mb