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Hi Daniel, look at this the Profinity eXact Fusion-tag system from BioRad* * the protease i fused to your protein and self activated by halides (F or I I think). Cleavage in on column. The principles is clever, now the cleavage conditions may not suit to your protein, but it seems to work. This is for expression and purification purposes only. Hope this helps -- Pascal F. Egea, PhD Assistant Professor UCLA, David Geffen School of Medicine Department of Biological Chemistry 314 Biomedical Sciences Research Building office (310)-983-3515 lab (310)-983-3516 email [log in to unmask]