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Dear James What size are your drops? If it takes that long for crystals to grow, it is very likely that something happens to the protein that stopped nucleation previously... it generally means some form of protein degradation. I would start by investigating exactly what you have on the crystals. If you still have crystals, run one on a gel and if the MW seems different, you can try to investigate further. It might mean getting a new, shorter, more "crystallizable" construct. Good luck Paula Salgado 2009/10/11 james09 pruza <[log in to unmask]> > Dear crystallographers, > > Sorry for the non-ccp4 query. I am new to this field and need some > suggestions. My question is, why some protein takes longer time to > crystallize, say 6-8 months, and it is the only condition to get the > crystals.? What are the ways to get the crystals faster. > > The crystal appears with 60% of 2-Methyl, 2-4 Pentane Diol and only at 4 > degree with very low concentration of NaCl. I have got some of the > sugggestions earlier from the CCP4-discussion board for microseeding, but it > did not work. > > All suggestions from the experts are welcome. > > Thanks. > James >