matlabmatrix=load('matrixname.mat'); However, I'm not sure what you want to do with it in matlab. Be aware that almost no two pieces of software use the same coordinate conventions and so using these matrices directly for another purpose outside of FSL is very tricky. All the best, Mark On 30 Sep 2009, at 06:49, Xi Liang wrote: > I want to load the transform matrix generated by FLIRT to Matlab, > how should I > convert it? >