Lower the concentration did help a little bit. I didn't the 6 mg/ml setups, but still got some skins.
You are right the skins are more likely to find in the condition of PEGs...but also find in other conditions.
Lower the conc. of PEGs is a good advice, and I'll try it. Thanks.
Shu
On Sun, Sep 6, 2009 at 10:29 AM, Jeremiah Farelli
<[log in to unmask]> wrote:
This happened to me previously, and the only thing that worked for me was to
lower the protein concentration. I was setting my protein up at 10 mg/mL,
only to find skins on nearly all the drops. I would damage the crystals
trying to get them out of the drops... I lowered my protein concentration to
7.5 mg/mL... the crystals still grew, and the skin didn't.
This seems to happen a lot when you set up crystals with PEGs, so you might
be able to lower the concentration of PEG as well.
