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Hi, all. I'm doing the crystallization screening for my protein. In over 1/2 of my hanging drops, there were skins formed on the surface of the drop. So I switched to use microbatch, and again almost 4/5 drops under the oil were skins at the interface. I tried to add additives, like detergents, octyl-beta-glucoside in the protein solution, but that didn't help. Is it because my protein is membrane assiociate protein or the hydrophobity of this protein is too high? I do need help and thanks a lot! Yours, Shu Xu