 |
 |
Mike England wrote: > Hi all, > > I will appreciate your comments on the following case: > > I have two datasets from the same or identical crystals. Initially, I > refine a structure against the first data set and later on switch to > another dataset for further refinements. > Do you think, my Rfree will be biased as Rfree reflections in second > dataset may be in fact Rwork reflections in previous datasets ? > > Thanks in advance, > > Mike > > > Yes [- it will be biased, but why arent preserving the same Rfree set from crystal 1 into the crystal 2 assignmenmt, only selecting new free reflections at the higher resolution data from crystal 2 If you are running scala or import scaled data t make an mtz file there is an option: Click Ensure Free R and Copy freeR from another mtz Eleanor