Hi - I have a group of 21 patients who underwent two fMRI sessions before and after treatment. I would like to compare activation before and after treatment on patient-by-patient basis and then do a higher level regression analysis to see whether change in group activation predicts behavioural improvement. For the within subject analysis I am using a higher level t-test comparing the two before and two after fMRI sessions. My questions is: Should I use a fixed effect analysis for this step? Also, for the higher level (third level) analysis that includes the whole group, should I use the COPEs as inputs? Hope this makes sense. Thanks - Julius