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Thanks Tom I do not get namefile_all_fast_firstseg.nii.gz I do not get the volume of the hippocampus again perform the steps, step 1: run_first_all image.nii.gz 4 output ( I use the number 4 in boundary_threshold that in this instance for example , but this has something to do with http://www.fmrib.ox.ac.uk/fsl/first/cma_subcortical_label.html ) step 2: output...: output_L_Accu.vtk output_L_Amyg.vtk output_L_Hip.vtk .. (but all archives extension = .vtk & .bvars and a single file nii.gz) output_to_std.nii.gz what is the error? Thank you very much for your time, Yours sincerely, Nico FSL 4.1.2 _________________________________________________________________ Mes de la amistad, disfrutalo con MSN Amor y Amistad http://match.ar.msn.com/channel/index.aspx?trackingid=1056241