Hello,
I'll add that when I get back (assuming I haven't forgotten by then!).
Wayne
On Wed, 12 Aug 2009, Kaare Teilum wrote:
> Dear all
>
> We have a titration series that we wanted to analyze with the Follow Shift
> Changes module.
>
> As the binding event has a low Kd we simply wanted to fit the chemical shift
> changes to:
>
> dw = dwMAX * fractionBound = dwMAX * [ligand]/(Kd+[ligand])
>
> The closest implementation of this equation is Ax/(1+Ax) where A = Ka. This
> however requires that the chemical shift change you observe is normalized to
> 1 - and I guess that this is rarely the case.
>
> The equation would be much more useful if it was modified to:
>
> B*Ax/(1+Ax) {A=Ka; B = dwMAX}
>
> or
>
> B*x/(A+x) {A=Kd; B = dwMAX}
>
> Cheers,
> Kaare
>
