 |
 |
We have unsuccessfully used in the past (by that I mean less than stellar incorporation) the Gibco/Invitrogen Sf900-SFM w/o Met/Cys media. Then we saw Karin Reinisch's paper (Dong et al, Immunity, 2009) and they used Expression Systems' ESF921. This protocol works for us. Engin On 8/18/09 2:49 PM, Brad Bennett wrote: > Hi Riya- > Expression Systems has a protocol for SeMet incorporation (Click on > the "Selenomethionine Incorporation" pdf file at > http://www.expressionsystems.com/?mvcTask=documents) and they > advertise that they do just about anything you would want to do with > insect cells (see > http://www.expressionsystems.com/?mvcTask=contractProduction), > including expression of recombinant protein in insect cells. I promise > that I am not an employee or a shareholder of this company. Just used > their products and I was looking for a SeMet protocol recently, too, > and happen to find theirs online. You can contact me off the board if > you want more details. > > HTH- > Brad > > On Tue, Aug 18, 2009 at 5:09 PM, riya doreen <[log in to unmask] > <mailto:[log in to unmask]>> wrote: > > Hi everyone, > I am looking for a method for expressing selenomethionine labeled > protein in insect cells. Can anyone point me to a suitable > protocol ? Additionally, is anyone aware of companies that may > provide this service for a fee ? > Thanks > > -- Engin Özkan Post-doctoral Scholar Laboratory of K. Christopher Garcia Howard Hughes Medical Institute Dept of Molecular and Cellular Physiology 279 Campus Drive, Beckman Center B173 Stanford School of Medicine Stanford, CA 94305 ph: (650)-498-7111