Hi everybody,
I really apologize to every members of CCP4BB for not to give attention to my addressing phrase. It is truly misleading. I swear not to make such bad mistake in future. I am really ashamed for such a deed.
Sincerely
Debajyoti Dutta
On Fri, 21 Aug 2009 21:29:23 +0530 wrote
>Dear Sir,
Please don't assume that all crystallographers are male.
---- Original message ----
>Date: Thu, 20 Aug 2009 16:45:55 -0000
>From: Debajyoti Dutta <[log in to unmask]>
>Subject: Re: [ccp4bb] cyseteine modification
>To: [log in to unmask]
>
> Dear Sir,
>
> Thank you all who have replied. I jast want to
> enquire that if there is any option in coot to
> introduce the OH bond with Cys residue as well as to
> introduce the secondary peptide like bond between
> carboxylic carbon with amino group of lys.
>
> Sincerely
> Debajyoti Dutta
>
> On Wed, 19 Aug 2009 20:58:44 +0530 wrote
> >
>
> Dear Debajyoti
>
> There is also the sulfenic acid species (-S-OH)
> which is actually
> the first oxidized form of sulfhydryls on the way to
> sulfonic acid. However
> sulfenic acids are very susceptible to further
> oxidation to sulfinic and sulphonic
> acids, and therefore need a protective chemical
> environment to remain stable.
>
> See for example some previous work of ours on
> sulfenic and
> sulfinic forms of active-site cysteines in
> glutathione reductase (Nature
> Structure Biology vol 5, 267-271, 1998) and the
> corrresponding pdb entries 1dnc
> and 1gsn.
>
> Best regards
>
> Savvas
>
> ----
> Savvas Savvides
> L-ProBE, Unit for Structural Biology
> Ghent University
> K.L. Ledeganckstraat 35
> 9000 Ghent, BELGIUM
> office: +32-(0)9-264.51.24 ;mobile:
> +32-(0)472-92.85.19
> Email: [log in to unmask]
> http://www.lprobe.ugent.be/xray.html
>
> From: CCP4 bulletin board
> [mailto:[log in to unmask]] On Behalf Of
> Debajyoti Dutta
> Sent: Wednesday, August 19, 2009 4:42 PM
> To: [log in to unmask]
> Subject: [ccp4bb] cyseteine modification
>
> Dear Sir,
>
> Is there any other oxidation states of cysteine
> other than cysteine sulphinic
> acid and cysteine sulphonic acid. In my protein, the
> cysteine molecule is
> definitely overoxidized but the electron density is
> not corresponding to the
> sulphinic or the sulphonic acid. The positive
> density looks as if it can
> accomodate only one oxygen atom and not more.
>
> Thank you for reply in advance.
>
> Sincerely
> Debajyoti Dutta
>
> >
> >E-mail message checked by Spyware Doctor
> (6.1.0.447)Database version:
> 6.13080http://www.pctools.com/spyware-doctor-antivirus/
>
> [IMG]
Phoebe A. Rice
Assoc. Prof., Dept. of Biochemistry & Molecular Biology
The University of Chicago
phone 773 834 1723
http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123
RNA is really nifty
DNA is over fifty
We have put them
both in one book
Please do take a
really good look
http://www.rsc.org/shop/books/2008/9780854042722.asp
