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Hi Sajid, the NADP /NAD binding sites are often "composed" of two sites: one site that is specific for the adenine/adenosine moiety and another site for the nicotinamide moiety. It can happen that you see the adenine in the density but not the nictoninamid tangling around. HTH Guenter > I have used NADP in my crystallisation condition; and the crystal diffracted well; I built the model completely and the r-factor and r-free are 0.26 and 0.29 respectively. The resolution is 2.3A. I am looking for NADP, in the binding region; I could see some density for NADP; But it is not continuous; there is no density for the middle phosphate group in fo-fc as well as 2fo-fc. Is it possible that NADP could hydrolyse? if so what could be the hydrolysed product > > > thank you > > sajid > > > > Looking for local information? Find it on Yahoo! Local http://in.local.yahoo.com/ > -- *********************************** Priv.Doz.Dr. Guenter Fritz Fachbereich Biologie Sektion Naturwissenschaften Universitaet Konstanz http://www.biologie.uni-konstanz.de/fritz Universitaetsstrasse 10 Postfach M665 D-78457 Konstanz e-mail: [log in to unmask] Phone Office: +49-(0)7531 88 3205 Phone Lab : +49-(0)7531 88 3733 Fax: +49-(0)7531 88 2966