Hi Riya, You'll need to install at least one of these programs for it to work. What kind of operating system have you got? Clustalw/2 is about the easiest to install and is available from here: ftp://ftp.ebi.ac.uk/pub/software/clustalw2/2.0.11/ Let me know if you need any further help with it. Best wishes, Ronan Ronan Keegan CCP4 Group riya doreen wrote: > Hi everyone, > > I am trying to run MrBUMP through the CCP4 6.1.1 but the program is > complaining that no multiple alignment programs like mafft, clustalw, > clustalw2, probcons, or t_coffee were found on the system. > > Will I need to install all these programs and will they need to be in > the CCP4 directory ? > Thanks