Roy We saw this at St James's Hospital in Leeds following an upgrade / maintenance to the pneumatic tube system. The cause was eventually diagnosed as being due to a non-functioning deceleration zone, so the pods were crashing into the buffers and this was haemolysing samples. My colleague Helen Shepherd had this as a poster at (I think) last year's Focus, but she's on leave so I can't give you the reference. Following repair of the deceleration zone, haemolysis rates returned to previous values. Best wishes, Steve Stephen R Goodall BSc MSc FRCPath Consultant Clinical Biochemist Clinical Biochemistry & Immunology Leeds Teaching Hospitals LS1 3EX UK Tel: 0113 392 3691 >>> Roy Fisher <[log in to unmask]> 07/08/2009 11:58 >>> Dear Colleagues, Since last weekend we have seen a large increase in the number of significantly haemolysed samples ( up to 30-40% ). Some patients are having to be bled three times before having a troponin T can be measured! Most come from A & E and MAU. We have eliminated most of the usual suspects, such as poor venepuncture technique, inappropriate use of vacutainer blood tubes, batches of tubes and handling/centrifugation in the lab. We have also run parallel clotted and heparin blood samples. We are now investigating the Air Tube system which was upgraded two weeks ago as it has been noticed that some lithium heparin bloods had bubbles at the top of the sample.. Has any other laboratory experienced such a problem? Is there any evidence that Air Tube systems can be implicated? Any other ideas welcome as we would prefer to have a relatively hassle free weekend. Many thanks Roy Roy Fisher Consultant Biochemist Royal Cornwall Hospital Tel 01872 252546----------------------------------------------------------------------------------------- The information in this e-mail and any attachments is confidential and is intended for the attention and use of the named addressee(s). It must not be disclosed to any other person without our authority. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient or are aware that this e-mail has been sent to you in error, you are not authorised to and must not disclose, copy, distribute, or retain this message or any part of it. We sweep all outgoing messages for the presence of computer viruses. However, we cannot accept any responsibility for any loss or damage to your systems due to viruses or malicious code not detected. The statements and opinions expressed in this message are those of the author and do not necessarily reflect those of the organisations within the Cornwall & Isles of Scilly Health Community. This email may be disclosed under the Freedom of Information Act 2000 or the Environmental Information Regulations 2004. ----------------------------------------------------------------------------------------- ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/