This should be fine. When you're doing the second level to combine across runs for a given subject, select "inputs are lower level FEAT directories" so that it will grab all of your copes from the 1st level. If you do this, your second level analysis will have outputs pertaining to cope1.feat through cope4.feat. Then, once you do the group analysis at the 3rd level, you can select "inputs are 3D cope images" -- for this, you would select the cope images from the 2nd level of each subject (e.g., subject1.gfeat/cope1.feat/stats/cope1.nii.gz). David -----Original Message----- From: FSL - FMRIB's Software Library [mailto:[log in to unmask]] On Behalf Of Melissa Farmer Sent: Sunday, July 26, 2009 12:50 PM To: [log in to unmask] Subject: [FSL] can't find the lower level copes I need My question has to do with feeding lower level copes to higher level analyses. I have checked through the archives and thought I found my answer, but I can't see how my existing cope files allow me to do what I want to do. At first level, each subject has multiple files that are fed into a second level: Grp EV1 EV2 "A" "B" Input 1 1 1 0 Input 2 1 1 0 Input 3 2 0 1 and 4 contrasts are formed: EV1 EV2 A 1 0 -A -1 0 A-B 1 -1 B-A -1 1 I want to feed the mean A contrast and the A-B contrasts up to separate higher level analyses. My assumption was that each of these contrasts should form a cope.feat file, so if I want to group multiple subjects on the first contrast (mean of A), I select cope1.feat files for the input at higher levels (and cope3.feat for the A-B contrast). However, the above design only has 2 copes in its output (within each cope in the stats subfolder, I do see cope1.nii.gz, cope2.nii.gz, etc), so it doesn't seem like I can feed each of the 4 contrasts up to higher levels. Previous archive posts indicate that feat files (not .nii.gz files) are to be fed into 2nd and higher levels. Can you please offer some direction as to what I'm missing here? Thanks, Melissa