Dear All:
Next week we are going to try some seleno-Met labeled crystals.
We checked the literature to try to find out the peak wavelength that has been used for SAD or MAD data collection. But they are slightly different ( may be 50 ev) in different papers.
I guess this is due to the discrepancy between the fluorescence scanning and the theoretical vaules of f' and f''.
When we collect the data, which wavelength should we use? Should we trust the scanning results?
Thanks a lot for your comments.
All the best,
Jerry McCully
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