With regard to the technical issues surrounding the assay of urine cortisol, the instrument upload/download code is the same as the serum assay (601) so be careful when processing a urine and serum cortisol with the same accession number since there is the possibility that the results will get mixed up; In Newport we get around this issue by giving all urine barcodes a 7 prefix.
 
Also Omnilab (Abbott middleware provider) took months to update their instrument driver software to permit the urine cortisol assay to use the same upload/download code as serum cortisol, but finally fixed it under Ticket number 200996.
 
I hope this info is of use to you.

Philip
 

Philip Waters Biomedical Scientist Grade 2 (IT and Quality) 
Biochemistry, Royal Gwent Hospital
Newport, NP20 2UB, UK
t       01633 234492
Fax  01633 234688
P Please consider the environment before printing this e-mail.

 
 

From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Jonathan Middle
Sent: 31 July 2009 11:06
To: [log in to unmask]
Subject: Re: Urine cortisol

List members might be interested in the following data:

In 2008 I performed a linearity exercise which involved mixing a 'normal' healthy urine with that of a Cushings patient in various ratios.

The comparative data (urine cortisol nmol/L) were:

% Cushings urine / Abbott Architect method mean n=5 labs / LCMSMS method mean n=10 labs

   0%   /     21 nmol/L   /    13 nmol/L
 25%   /   112 nmol/L   /     81 nmol/L
 75%   /   298 nmol/L   /   228 nmol/L
100%  /   389 nmol/L   /   297 nmol/L

Linearity was good for both method groups.

In the UK NEQAS Scheme, the current (dist 353) median bias against the ALTM for the Architect group (n=10) is -8.9% and of the LCMSMS group (n=13) is -18.0%

In a very recent recovery exercise (dist 353), the Architect group averaged 108.7% across three spiked pools, and the LCMSMS group averaged 100.9%.

Hope this helps

Jonathan Middle (Organiser UK NEQAS for Steroid Hormones)
 


On Thu, Jul 30, 2009 at 4:31 PM, Bart Ballieux <[log in to unmask]> wrote:
Dear colleagues,
 
Immunoassay of urinary free cortisol is one of the least standardized tests I know of and has inherent risk for false positive results. Only HPLC or LC/tandem MS will produce comparable results, which are much lower than for immunoassay (with or without extraction with dichloromethane). Since the Abbott Axsym uses an immunoassay without extraction it will be extremely sensitive for crossreactivity with various metabolites and conjugates. So, reference values won't help you very much.
 
Best regards,
 
Bart Ballieux

 B.E.P.B. Ballieux PhD
Clinical Biochemist
CKCL, E2-P
Leiden University Medical Centre
P.O.box 9600
2300RC  Leiden
Tel: +3171-5262165
Fax: +3171-5266753
email: [log in to unmask]
P Please consider the environment before printing this e-mail


From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Bouhtiauy, Ihssan (R4)
Sent: donderdag 30 juli 2009 15:22

To: [log in to unmask]
Subject: Urine cortisol

I will appreciate to hear from users of Abbott about their experience with the urine cortisol.  And also I will appreciate to have the reference values you are using.

Thanks

Ihssan

 

Dr. Ihssan Bouhtiauy, PhD, FCACB, FACB, MBA
nouveau courriel/ new email [log in to unmask]
Chef Clinique du département de Biochimie
Régie Régionale de la santé4
Edmundston, NB
Canada

 

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