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Dear colleagues, Immunoassay of urinary free cortisol is one of the least standardized tests I know of and has inherent risk for false positive results. Only HPLC or LC/tandem MS will produce comparable results, which are much lower than for immunoassay (with or without extraction with dichloromethane). Since the Abbott Axsym uses an immunoassay without extraction it will be extremely sensitive for crossreactivity with various metabolites and conjugates. So, reference values won't help you very much. Best regards, Bart Ballieux B.E.P.B. Ballieux PhD Clinical Biochemist CKCL, E2-P Leiden University Medical Centre P.O.box 9600 2300RC Leiden Tel: +3171-5262165 Fax: +3171-5266753 email: [log in to unmask] P Please consider the environment before printing this e-mail ________________________________ From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Bouhtiauy, Ihssan (R4) Sent: donderdag 30 juli 2009 15:22 To: [log in to unmask] Subject: Urine cortisol I will appreciate to hear from users of Abbott about their experience with the urine cortisol. And also I will appreciate to have the reference values you are using. Thanks Ihssan Dr. Ihssan Bouhtiauy, PhD, FCACB, FACB, MBA nouveau courriel/ new email [log in to unmask] <mailto:[log in to unmask]> Chef Clinique du département de Biochimie Régie Régionale de la santé4 Edmundston, NB Canada ------Avis de non-responsabilité------ Ce message électronique et tout fichier qui y est joint pourraient contenir des renseignements privilégiés ou confidentiels destinés uniquement à la personne ou à l'organisme nommé ci-dessus. Toute action entreprise relativement à ce message ainsi que toute reproduction, transmission ou diffusion partielle ou totale de celui-ci sont strictement défendues. Si vous avez reçu ce message électronique par erreur, veuillez en informer l'expéditeur et détruire le message immédiatement. Merci. -------------DISCLAIMER------------- This electronic transmission and any accompanying attachments may contain privileged or confidential information intended only for the use of the individual or organization named above. Any action related with this communication as well as any reproduction, transmission and/or dissemination in whole or in part is strictly prohibited. If you have received this communication in error please notify the sender and delete this email immediately. Thank you. ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/