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An external .mat file is used to save the relative orientations of the volumes in the 4D file. In SPM, if there is a .mat file, then the orientations should be read from it. Best regards, -John On Tue, 2009-06-02 at 13:22 -0700, Neil Chatterjee wrote: > I wanted to add one caveat because there is very sneaky problem with > realignment and 4D files. In 4D nifti files, all of the volumes share > the same header. So if you run something like realignment w/o > reslicing, which works by adjusting image headers relative to eachother, > you'll end up doing nothing. You can test it out by taking a 4D file, > rotating only one volume, and then looking at a different volume. > Perhaps things have changed in SPM8, but last I tried this both volumes > would be rotated. It's for this reason I prefer to keep my files in 3D, > even if it clutters up my filesystem. Just my $.02 ..... > > -Neil > > John Ashburner wrote: > > In the file selector, try changing the field that usually says "1" to > > something like 1:1000 > > > > Best regards, > > -John > > > > On Tue, 2009-06-02 at 11:50 -0700, Michael T Rubens wrote: > > > >> convert .imgs to 4D nifti. (see spm_config_3Dto4D) > >> > >> Cheers, > >> Michael > >> > >> On Tue, Jun 2, 2009 at 11:02 AM, Eric Mansfield > >> <[log in to unmask]> wrote: > >> Good Day, > >> I was wondering, does SPM8 support 4D .img files? I've tried > >> loading 4D .img > >> files into the realign function and it only loads the first > >> image. Is it possible for > >> me load my entire time series without breaking it into > >> individual 3D .img files? > >> > >> Thank you, > >> > >> Eric Mansfield > >> Duke Brain Imaging > >> and Analysis Center > >> > >> > >> > >> -- > >> Research Associate > >> Gazzaley Lab > >> Department of Neurology > >> University of California, San Francisco > >> > -- John Ashburner <[log in to unmask]>