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Dear Ram, > I have 16S subjects, each one with 3 run's. > Is it o,k on the FEAT.gui on the First-level analysis to leave the > initial > and main structural image tabs un-marked, using only MNI152 as > standard space? > This means not using the Ss high-res anatomy file. > How does that effect my first/higher levels? > what are the adv' / disadvantage? do you mean that you want to register the EPI images directly to the MNI template without using the individual subjects structural scans? I think that would be a bad idea. The contrast is very different in your EPI compared to in the MNI template, making that a difficult registration problem. Therefore you want as few degrees of freedom as possible for that registration, i.e. just the 6 associated with a rigid body transform. To register a structural image to the MNI template is much easier since they will have similar intensity contrasts. Hence, the better way to do things is EPI--6dof-->Structural--12(or more)dof-->MNI152. Good Luck Jesper