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will work fine but leave overnight. psp On Tue, Jun 2, 2009 at 5:36 PM, Jerry McCully <[log in to unmask]> wrote: > Dear All: > > Recent we expressed one protein using mammalian cells but this > protein was highly glycosylated (30% of the total molecular weight). > > We want to remove some carbohydrate by enzymatic digestion for the > convenience of crystallization. However, this protein tends to aggregate at > 37 degree. > > I am not sure whether EndoH and PNGase can work at 4 degree. > > Can anyone give some suggestion? > > Thanks a lot, > > Jerry McCully > > > > > > ________________________________ > Hotmail® has ever-growing storage! Don’t worry about storage limits. Check > it out. -- Pius S Padayatti Scientist, Polgenix, Inc. 11000 Cedar Ave, Suite 260 Cleveland, OH 44106 Phone: 216-658-4528 Fax: 216-658-4529