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My structure has a Cys that is solvent-exposed and an unidentified 'blob' of density which is connected to the sulfur of the Cys. The protein was crystallized in presence of DTT, and DTT does fit the density. However, I do not know if it is oxidized or not, as the density does not appear to accommodate the ring conformation of oxidized DTT. Does anyone know of an article that talks about the chemistry of DTT, reducing agents in crystallographic structures or have any suggestions as to what is going on? Thank you, Lynn