Hello Dr. Maguire

 

If I recall correctly, the LDH reaction on the Beckman is a NAD to NADH reaction in the presence of lactate and measured at 340.I do not suspect that hydroxyurea absorbs at 340 and the drug has a very short ˝ life.  Were there any other 340 kinetic assays ordered?

 

Joe Indovina

 

 

Dr John M. Land
Clinical Director Biochemical Medicine
UCLH NHS Foundation Trust
Neurometabolic Unit Box 105
National Hospital
Queen Square
London WC1N 3BG

44-(0)20-7829-8768

-----Original Message-----
From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Sean Maguire
Sent: 09 June 2009 13:27
To: [log in to unmask]
Subject: Interference with LDH assay

Dear colleagues,

 

Can I ask if anyone has experienced interference with their LDH assay (we use the Beckman Coulter DX) from anti-cancer drugs?

We had a recent experience of a patient with an artefactually low LDH who had recently started treatment with hydroxyurea.

 

Many thanks   

 

Dr. Sean Maguire,

Biochemistry Laboratory,

Mater Hospital,

Dublin.

------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/

------------------------------------------------------------------------

This email is confidential and is intended solely for the person or

Entity to whom it is addressed.  If this is not you, please forward the

Message to [log in to unmask]  We have scanned this email

before sending it, but cannot guarantee that malicious software is

absent and we shall carry no liability in this regard.

We advise that information intended to be kept confidential should not

Be sent by email.  We also advise that health concerns should be

Discussed with a medical professional in person or by telephone.

NHS Direct can also provide advice.  We shall not be liable for any

failure to follow this advice. University College London Hospitals NHS

Foundation Trust (UCLH).

------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/